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Product Name
MTF2 antibody
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Description
MTF2 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, HepG2 cells, mouse brain tissue, mouse ES cells and 293 cells, mouse skeletal muscle tissue, rat lymph tissue. Positive IP detected in mouse skeletal muscle tissue. Positive IF detected in mouse ES cells. Positive IHC detected in human brain tissue. Observed molecular weight by Western-blot: 45-46kd,55kd,60-61kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
dJ976O13.2 antibody; hPCl2 antibody; M96 antibody; MTF2 antibody; PCL2 antibody; Polycomb like protein 2 antibody; RP5 976O13.1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MTF2 recombinant protein (Accession Number: NM_001164392). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:112772(MTF2 antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human brain using Catalog No:112772(MTF2 antibody) at dilution of 1:50 (under 40x lens)
IF result of mouse ES cell From Kevin Corbit.
IP Result of anti-MTF2 (IP:Catalog No:112772, 4ug; Detection:Catalog No:112772 1:300) with mouse skeletal muscle tissue lysate 4000ug.
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Background
MTF2 represents metal response element binding transcription factor 2
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References
- Hunkapiller J, Shen Y, Diaz A. Polycomb-like 3 promotes polycomb repressive complex 2 binding to CpG islands and embryonic stem cell self-renewal. PLoS genetics. 8(3):e1002576. 2012.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"