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Product Name
MSH6 antibody
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Description
MSH6 Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive IHC detected in human spleen tissue, human colon tissue, human heart tissue, human kidney tissue, human ovary tissue, human testis tissue. Positive WB detected in HEK-293 cells, COLO 320 cells, HeLa cells. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 150-160 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
G/T mismatch binding protein antibody; GTBP antibody; GTMBP antibody; hMSH6 antibody; HNPCC5 antibody; HSAP antibody; MSH6 antibody; MutS alpha 160 kDa subunit antibody; mutS homolog 6 (E. coli) antibody; p160 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MSH6 recombinant protein (Accession Number: NM_000179). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:112869(MSH6 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human spleen using Catalog No:112869(MSH6 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using MSH6 antibody Catalog No:112869 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-MSH6 (IP:Catalog No:112869, 4ug; Detection:Catalog No:112869 1:600) with HEK-293 cells lysate 1120ug.
Immunohistochemistry of paraffin-embedded human colon tissue slide using Catalog No:112869(MSH6 Antibody) at dilution of 1:200 (under 40x lens)
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Background
MSH6, also named as DNA mismatch repair protein Msh6 or GTMBP, is a 1360 amino acid protein, which contains one PWWP domain and belongs to the DNA mismatch repair MutS family. MSH6 localizes in the nucleus and is a component of the post-replicative DNA mismatch repair system. Msh2 and Msh6 form a protein complex required to repair mismatches generated during DNA replication.
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