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Product Name
MSH6 antibody
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Description
MSH6 Mouse Monoclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells, HepG2 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 160 kDa
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Tested applications
ELISA, WB, IP, IF, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
G/T mismatch binding protein antibody; GTBP antibody; GTMBP antibody; hMSH6 antibody; HNPCC5 antibody; HSAP antibody; MSH6 antibody; MutS alpha 160 kDa subunit antibody; mutS homolog 6 (E. coli) antibody; p160 antibody
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Isotype
Mouse IgG2a
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Preparation
This antibody was obtained by immunization of MSH6 recombinant protein (Accession Number: NM_000179). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:50-1:500
IF: 1:50-1:500
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:107431(MSH6 Antibody) at dilution of 1:1000
IP Result of anti-MSH6 (IP:Catalog No:107431, 4ug; Detection:Catalog No:107431 1:600) with HEK-293 cells lysate 1800ug.
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:107431(MSH6 Antibody) at dilution of 1:800 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:107431(MSH6 Antibody) at dilution of 1:800 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:107431(MSH6 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
MSH6, also named as DNA mismatch repair protein Msh6 or GTMBP, is a 1360 amino acid protein, which contains one PWWP domain and belongs to the DNA mismatch repair MutS family. MSH6 localizes in the nucleus and is a component of the post-replicative DNA mismatch repair system. Msh2 and Msh6 form a protein complex required to repair mismatches generated during DNA replication.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"