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MRPS35 antibody

Cat.#: 112858

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Product Information

  • Product Name
    MRPS35 antibody
  • Documents
  • Description
    MRPS35 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, human heart tissue. Positive IP detected in HeLa cells. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 37 kDa
  • Tested applications
    ELISA, WB, IF, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    DKFZp762P093 antibody; HDCMD11P antibody; MDS023 antibody; MRP S28 antibody; MRP S35 antibody; MRPS28 antibody; MRPS35 antibody; PSEC0213 antibody; S28mt antibody; S35mt antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of MRPS35 recombinant protein (Accession Number: NM_021821). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IF: 1:10-1:100

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112858(MRPS35 antibody) at dilution of 1:2000

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112858(MRPS35 antibody) at dilution of 1:2000

    Immunofluorescent analysis of HepG2 cells, using MRPS35 antibody Catalog No:112858 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of HepG2 cells, using MRPS35 antibody Catalog No:112858 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    IP Result of anti-MRPS35 (IP:Catalog No:112858, 4ug; Detection:Catalog No:112858 1:1000) with HeLa cells lysate 1200ug.

    IP Result of anti-MRPS35 (IP:Catalog No:112858, 4ug; Detection:Catalog No:112858 1:1000) with HeLa cells lysate 1200ug.

  • Background
    Mammalian mitochondrial ribosomes (mitoribosomes) are responsible for protein synthesis within the mitochondrion. The mitoribosomes are composed of a 4:1 ratio of protein to RNA, with the proteins forming two subunits, the 28S subunit and the 39S subunit. Across species, the proteins that make up the mitoribosome subunits vary greatly in sequence, preventing easy recognition by sequence homology. MRPS35 (mitochondrial ribosomal protein S35), also known as MDS023, MRPS28 or HDCMD11P, is a 323 amino acid protein that localizes to the mitochondrion, where it exists as a component of the 28S ribosomal subunit and works in conjunction with other MRPs to mediate protein synthesis. Existing as two alternatively spliced isoforms, MRPS35 is encoded by a gene located on human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome. This antibody is a rabbit polyclonal antibody raised against the full length MRPS35 protein. It specifically reacts with the 37kd MRPS35protein.
  • References
    • Almajan ER, Richter R, Paeger L. AFG3L2 supports mitochondrial protein synthesis and Purkinje cell survival. The Journal of clinical investigation. 122(11):4048-58. 2012.
    • Dogan SA, Pujol C, Maiti P. Tissue-specific loss of DARS2 activates stress responses independently of respiratory chain deficiency in the heart. Cell metabolism. 19(3):458-69. 2014.
    • Richman TR, Ermer JA, Davies SM. Mutation in MRPS34 compromises protein synthesis and causes mitochondrial dysfunction. PLoS genetics. 11(3):e1005089. 2015.
    • Lagouge M, Mourier A, Lee HJ. SLIRP Regulates the Rate of Mitochondrial Protein Synthesis and Protects LRPPRC from Degradation. PLoS genetics. 11(8):e1005423. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"