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Product Name
MRPS27 antibody
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Description
MRPS27 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, A431 cells, human heart tissue, human skeletal muscle tissue. Positive IP detected in A431 cells. Positive IHC detected in human kidney tissue, human lung tissue, human spleen tissue, human testis tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 48kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ21764 antibody; FLJ23348 antibody; KIAA0264 antibody; MRP S27 antibody; MRPS27 antibody; S27mt antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MRPS27 recombinant protein (Accession Number: BC030521). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112854(MRPS27 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human kidney using Catalog No:112854(MRPS27 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using MRPS27 antibody Catalog No:112854 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-MRPS27 (IP:Catalog No:112854, 3ug; Detection:Catalog No:112854 1:1000) with A431 cells lysate 1520ug.
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Background
Genes encoding PPR (pentatricopeptide repeat)-containing proteins constitute one of the largest gene families in plants. The majority of these proteins are predicted to target organelles and to bind to RNA. Strikingly, there is a dearth of these proteins in mammals, although genomic searches reveal six candidates, all of which are also predicted to target the mitochondrion. One of these proteins, MRPS27is participated in transcription and translation respectively. [PMID:18939947] MRPS27is a component of the small subunit of the mitochondrial ribosome that is encoded by the nuclear genome [PMID: 11279123].
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References
- Davies SM, Lopez Sanchez MI, Narsai R. MRPS27 is a pentatricopeptide repeat domain protein required for the translation of mitochondrially encoded proteins. FEBS letters. 586(20):3555-61. 2012.
- Surovtseva YV, Shadel GS. Transcription-independent role for human mitochondrial RNA polymerase in mitochondrial ribosome biogenesis. Nucleic acids research. 41(4):2479-88. 2013.
- Antonicka H, Sasarman F, Nishimura T, Paupe V, Shoubridge EA. The mitochondrial RNA-binding protein GRSF1 localizes to RNA granules and is required for posttranscriptional mitochondrial gene expression. Cell metabolism. 17(3):386-98. 2013.
- Almajan ER, Richter R, Paeger L. AFG3L2 supports mitochondrial protein synthesis and Purkinje cell survival. The Journal of clinical investigation. 122(11):4048-58. 2012.
- Richman TR, Davies SM, Shearwood AM. A bifunctional protein regulates mitochondrial protein synthesis. Nucleic acids research. 42(9):5483-94. 2014.
- Dalla Rosa I, Durigon R, Pearce SF. MPV17L2 is required for ribosome assembly in mitochondria. Nucleic acids research. 42(13):8500-15. 2014.
- Antonicka H, Shoubridge EA. Mitochondrial RNA Granules Are Centers for Posttranscriptional RNA Processing and Ribosome Biogenesis. Cell reports. 2015.
- Tu YT, Barrientos A. The Human Mitochondrial DEAD-Box Protein DDX28 Resides in RNA Granules and Functions in Mitoribosome Assembly. Cell reports. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"