-
Product Name
MRPS22 antibody
- Documents
-
Description
MRPS22 Rabbit Polyclonal antibody. Positive IP detected in Jurkat cells. Positive WB detected in Jurkat cells, mouse liver tissue, mouse spleen tissue, rat spleen tissue. Positive IHC detected in human breast cancer tissue. Observed molecular weight by Western-blot: 38 kDa
-
Tested applications
ELISA, IHC, WB, IP
-
Species reactivity
Human, Mouse; other species not tested.
-
Alternative names
C3orf5 antibody; COXPD5 antibody; GIBT antibody; GK002 antibody; MRP S22 antibody; MRPS22 antibody; RPMS22 antibody; S22mt antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of MRPS22 recombinant protein (Accession Number: NM_020191). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
IHC: 1:20-1:200
-
Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:112849(MRPS22 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:112849(MRPS22 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-MRPS22 (IP:Catalog No:112849, 4ug; Detection:Catalog No:112849 1:300) with Jurkat cells lysate 2400ug.
-
Background
MRPS22, also named as C3orf5, RPMS22 and S22mt, is a component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins. Defects in MRPS22 are the cause of combined oxidative phosphorylation deficiency type 5 (COXPD5). MRPS22 is a 360-amino-acid protein that has been found in a limited number of species, having no ortholog in fungi or prokaryotes. The exact location of MRPS22 in the three dimensional structure of the ribosome and its exact function are currently unknown. Recently, a MRPS22 defect was shown to strongly hamper assembly of the small ribosomal subunit, whereas assembly of the large subunit and part of the small subunit were only mildly affected.(PMID:21189481)
-
References
- Wanschers BF, Szklarczyk R, Pajak A. C7orf30 specifically associates with the large subunit of the mitochondrial ribosome and is involved in translation. Nucleic acids research. 40(9):4040-51. 2012.
- Yagi M, Uchiumi T, Takazaki S. p32/gC1qR is indispensable for fetal development and mitochondrial translation: importance of its RNA-binding ability. Nucleic acids research. 40(19):9717-37. 2012.
- Antonicka H, Sasarman F, Nishimura T, Paupe V, Shoubridge EA. The mitochondrial RNA-binding protein GRSF1 localizes to RNA granules and is required for posttranscriptional mitochondrial gene expression. Cell metabolism. 17(3):386-98. 2013.
- Tucker EJ, Wanschers BF, Szklarczyk R. Mutations in the UQCC1-interacting protein, UQCC2, cause human complex III deficiency associated with perturbed cytochrome b protein expression. PLoS genetics. 9(12):e1004034. 2013.
- Zurita Rendón O, Silva Neiva L, Sasarman F, Shoubridge EA. The arginine methyltransferase NDUFAF7 is essential for complex I assembly and early vertebrate embryogenesis. Human molecular genetics. 23(19):5159-70. 2014.
- Dalla Rosa I, Durigon R, Pearce SF. MPV17L2 is required for ribosome assembly in mitochondria. Nucleic acids research. 42(13):8500-15. 2014.
- Rajala N, Gerhold JM, Martinsson P, Klymov A, Spelbrink JN. Replication factors transiently associate with mtDNA at the mitochondrial inner membrane to facilitate replication. Nucleic acids research. 42(2):952-67. 2014.
- Antonicka H, Shoubridge EA. Mitochondrial RNA Granules Are Centers for Posttranscriptional RNA Processing and Ribosome Biogenesis. Cell reports. 2015.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"