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Product Name
MRPS18B antibody
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Description
MRPS18B Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, HepG2 cells, human liver tissue, MCF7 cells, mouse liver tissue, rat heart tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human breast cancer tissue, human liver tissue. Observed molecular weight by Western-blot: 29 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
C6orf14 antibody; DKFZp564H0223 antibody; HSPC183 antibody; HumanS18a antibody; MRP S18 2 antibody; MRP S18 b antibody; MRPS18 2 antibody; Mrps18 b antibody; MRPS18B antibody; PTD017 antibody; S18amt antibody; S18mt b antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MRPS18B recombinant protein (Accession Number: NM_014046). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112846(MRPS18B antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:112846(MRPS18B antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:112846(MRPS18B Antibody) at dilution of 1:50 andand Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-MRPS18B (IP:Catalog No:112846, 4ug; Detection:Catalog No:112846 1:1000) with HeLa cells lysate 2800ug.
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Background
MRPS18B, also named as S18mt-b, MRP-S18-2 and C6orf14, belongs to the ribosomal protein S18P family. It is a component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins. This antibody recognizes specifically the 29 kd MRPS18B protein.
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References
- Wydro M, Bobrowicz A, Temperley RJ, Lightowlers RN, Chrzanowska-Lightowlers ZM. Targeting of the cytosolic poly(A) binding protein PABPC1 to mitochondria causes mitochondrial translation inhibition. Nucleic acids research. 38(11):3732-42. 2010.
- Dennerlein S, Rozanska A, Wydro M, Chrzanowska-Lightowlers ZM, Lightowlers RN. Human ERAL1 is a mitochondrial RNA chaperone involved in the assembly of the 28S small mitochondrial ribosomal subunit. The Biochemical journal. 430(3):551-8. 2010.
- Kolanczyk M, Pech M, Zemojtel T. NOA1 is an essential GTPase required for mitochondrial protein synthesis. Molecular biology of the cell. 22(1):1-11. 2011.
- Surovtseva YV, Shutt TE, Cotney J. Mitochondrial ribosomal protein L12 selectively associates with human mitochondrial RNA polymerase to activate transcription. Proceedings of the National Academy of Sciences of the United States of America. 108(44):17921-6. 2011.
- Rorbach J, Gammage PA, Minczuk M. C7orf30 is necessary for biogenesis of the large subunit of the mitochondrial ribosome. Nucleic acids research. 40(9):4097-109. 2012.
- Wanschers BF, Szklarczyk R, Pajak A. C7orf30 specifically associates with the large subunit of the mitochondrial ribosome and is involved in translation. Nucleic acids research. 40(9):4040-51. 2012.
- Surovtseva YV, Shadel GS. Transcription-independent role for human mitochondrial RNA polymerase in mitochondrial ribosome biogenesis. Nucleic acids research. 41(4):2479-88. 2013.
- Antonicka H, Sasarman F, Nishimura T, Paupe V, Shoubridge EA. The mitochondrial RNA-binding protein GRSF1 localizes to RNA granules and is required for posttranscriptional mitochondrial gene expression. Cell metabolism. 17(3):386-98. 2013.
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