Product Name

MRFAP1 antibody

Documents

Description

MRFAP1 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells. Positive IHC detected in human gliomas tissue. Observed molecular weight by Western-blot: 15kd

Tested applications

ELISA, WB, IHC

Species reactivity

Human; other species not tested.

Alternative names

MRFAP1 antibody; PAM14 antibody; PGR1 antibody; Protein PGR1 antibody

Immunogen

Human MRFAP1 Recombinant protein (GST tag)

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of MRFAP1 recombinant protein (Accession Number: NM_001272053). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:200-1:1000

IHC: 1:20-1:200

Validations

Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:112803(MRFAP1 antibody) at dilution of 1:400

Immunohistochemical of paraffin-embedded human gliomas using Catalog No:112803(MRFAP1 antibody) at dilution of 1:50 (under 10x lens)

Background

MRFAP1, also named as PAM14 and PGR1, is a 14 kDa protein which associated with MRG. It is found in a complex composed of MORF4L1, MRFAP1 and RB1. MRFAP1 interacts via its N-terminus with MORF4L1. And it interacts with CSTB and MORF4L2.

References

Liu X, Wang Y, Zhu H. Interaction of Sedlin with PAM14. Journal of cellular biochemistry. 109(6):1129-33. 2010.
Kirkwood KJ, Ahmad Y, Larance M, Lamond AI. Characterization of native protein complexes and protein isoform variation using size-fractionation-based quantitative proteomics. Molecular & cellular proteomics : MCP. 12(12):3851-73. 2013.
Liao H, Liu XJ, Blank JL. Quantitative proteomic analysis of cellular protein modulation upon inhibition of the NEDD8-activating enzyme by MLN4924. Molecular & cellular proteomics : MCP. 10(11):M111.009183. 2011.

MRFAP1 antibody

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