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Product Name
MPI antibody
- Documents
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Description
MPI Rabbit Polyclonal antibody. Positive IF detected in NIH/3T3 cells. Positive IP detected in mouse brain tissue. Positive WB detected in human brain tissue, mouse brain tissue. Observed molecular weight by Western-blot: 43 kDa
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Tested applications
ELISA, WB, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ39201 antibody; Mannose 6 phosphate isomerase antibody; mannose phosphate isomerase antibody; MPI antibody; Phosphohexomutase antibody; Phosphomannose isomerase antibody; PMI antibody; PMI1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MPI recombinant protein (Accession Number: NM_002435). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IF: 1:50-1:500
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:112744(MPI antibody) at dilution of 1:1200
IP Result of anti-MPI (IP:Catalog No:112744, 4ug; Detection:Catalog No:112744 1:1000) with mouse brain tissue lysate 4000ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed NIH/3T3 cells using Catalog No:112744(MPI Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
MPI(mannose-6-phosphate isomerase) is also named as PMI1 and belongs to the mannose-6-phosphate isomerase type 1 family. It is a zinc metalloenzyme that catalyzes the reversible isomerization of mannose-6-phosphate and fructose-6-phosphate. MPI plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. This proterin has 2 isoforms produced by alternative splicing.
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