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Product Name
MOCOS antibody
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Description
MOCOS Rabbit Polyclonal antibody. Positive IP detected in HepG2 cells. Positive WB detected in HepG2 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human liver tissue. Observed molecular weight by Western-blot: 98 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ20733 antibody; HMCS antibody; MCS antibody; MOCo sulfurase antibody; MOCOS antibody; molybdenum cofactor sulfurase antibody; MOS antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of M℃OS recombinant protein (Accession Number: NM_017947). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:112724(MocOS antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human liver using Catalog No:112724(MocOS antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:112724(MocOS antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-MocOS (IP:Catalog No:112724, 4ug; Detection:Catalog No:112724 1:300) with HepG2 cells lysate 4000ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:112724(MocOS Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
MocOS(Molybdenum cofactor sulfurase) is also named as MCS, MOS, hMCS and belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. It catalyzes the removal of elemental sulfur from cysteine to produce alanine. Sulfation of molybdenum is essential for xanthine dehydrogenase (XDH) and aldehyde oxidase (ADO) enzymes in which molybdenum cofactor is liganded by 1 oxygen and 1 sulfur atom in active form. Defects in MocOS are the cause of xanthinuria type 2 (XU2)(PMID:17368066).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"