-
Product Name
MMP8 antibody
- Documents
-
Description
MMP8 Rabbit Polyclonal antibody. Positive IHC detected in human placenta tissue, human kidney tissue, human ovary tissue, human spleen tissue, human testis tissue. Positive WB detected in HepG2 cells, Jurkat cells, mouse liver tissue. Positive IP detected in mouse liver tissue. Observed molecular weight by Western-blot: 70 kDa
-
Tested applications
ELISA, WB, IHC, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
CLG1 antibody; HNC antibody; Matrix metalloproteinase 8 antibody; MMP 8 antibody; MMP8 antibody; Neutrophil collagenase antibody; PMNL CL antibody; PMNL collagenase antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of MMP8 recombinant protein (Accession Number: NM_002424). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
-
Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:112708(MMP8 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human placenta using Catalog No:112708(MMP8 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human placenta using Catalog No:112708(MMP8 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-MMP8 (IP:Catalog No:112708, 4ug; Detection:Catalog No:112708 1:500) with mouse liver tissue lysate 3600ug.
-
Background
MMP8(Matrix metalloproteinase-8) an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells(PMID:15367487). Purified human neutrophil MMP-8 with proeMMP-8 at 75 KDa and active enzyme at 65 KDa and amniochorion proenzyme and active enzyme at 65 and 50 KDa,respectively(PMID:15042023).
-
References
- Mousa AA, Cappello RE, Estrada-Gutierrez G. Preeclampsia is associated with alterations in DNA methylation of genes involved in collagen metabolism. The American journal of pathology. 181(4):1455-63. 2012.
- Vagima Y, Zauberman A, Levy Y. Circumventing Y. pestis Virulence by Early Recruitment of Neutrophils to the Lungs during Pneumonic Plague. PLoS pathogens. 11(5):e1004893. 2015.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"