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Product Name
MMP2 antibody
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Description
MMP2 Rabbit Polyclonal antibody. Positive WB detected in MCF7 cells, A375 cells, A431 cells, HepG2 cells, HT-1080 cells, MDA-MB-453s cells, mouse heart tissue, mouse liver tissue, mouse lung tissue, rat heart tissue, SGC-7901 cells. Positive IP detected in NIH/3T3 cells. Positive IHC detected in human gliomas tissue, human breast cancer tissue, human heart tissue, human oesophagus tissue, human placenta tissue. Observed molecular weight by Western-blot: 72 kDa, 60 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat, Zebrafish; other species not tested.
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Alternative names
72 kDa gelatinase antibody; 72 kDa type IV collagenase antibody; CLG4 antibody; CLG4A antibody; Gelatinase A antibody; Matrix metalloproteinase 2 antibody; MMP 2 antibody; MMP II antibody; MMP2 antibody; MONA antibody; TBE 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MMP2 recombinant protein (Accession Number: NM_004530). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:112700(MMP2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:112700(MMP2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:112700(MMP2 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-MMP2 (IP:Catalog No:112700, 4ug; Detection:Catalog No:112700 1:300) with NIH/3T3 cells lysate 1200ug.
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Background
MMP2, also named as CLG4A, Gelatinase Am, TBE-1 and PEX, belongs to the peptidase M10A family. It is ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. MMP2 contributes to myocardial oxidative stress by regulating the activity of GSK3beta. It cleaves GSK3beta in vitro. MMP2 can be cleaved into PEX chain(~60kd).Western blot analysis showed that the 72 kDa and 62 kDa proteinase activities were pro-MMP2 and the active enzyme,respectively(PMID:11112697).
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References
- Huang D, Du X, Yuan R. Rock2 promotes the invasion and metastasis of hepatocellular carcinoma by modifying MMP2 ubiquitination and degradation. Biochemical and biophysical research communications. 453(1):49-56. 2014.
- Yang X, Li Z, Ma Y. Human umbilical cord mesenchymal stem cells promote carcinoma growth and lymph node metastasis when co-injected with esophageal carcinoma cells in nude mice. Cancer cell international. 14(1):93. 2014.
- Yang X, Zhang H, Wang J, Zhang Z, Li C. Puerarin decreases bone loss and collagen destruction in rats with ligature-induced periodontitis. Journal of periodontal research. 50(6):748-57. 2015.
- Yang Y, He G, Xu W, Liu X. ENaC mediates human extravillous trophblast cell line (HTR8/SVneo) invasion by regulating levels of matrix metalloproteinase 2 (MMP2). Placenta. 36(5):587-93. 2015.
- Huang W, Liu J, Feng X. DLC-1 induces mitochondrial apoptosis and epithelial mesenchymal transition arrest in nasopharyngeal carcinoma by targeting EGFR/Akt/NF-κB pathway. Medical oncology (Northwood, London, England). 32(4):115. 2015.
- Qiao J, Cui SJ, Xu LL. Filamin C, a dysregulated protein in cancer revealed by label-free quantitative proteomic analyses of human gastric cancer cells. Oncotarget. 6(2):1171-89. 2015.
- Yao M, Wang X, Wang X, Zhang T, Chi Y, Gao F. The Notch pathway mediates the angiotensin II-induced synthesis of extracellular matrix components in podocytes. International journal of molecular medicine. 36(1):294-300. 2015.
- Zou Y, Li J, Chen Z. miR-29c suppresses pancreatic cancer liver metastasis in an orthotopic implantation model in nude mice and affects survival in pancreatic cancer patients. Carcinogenesis. 36(6):676-84. 2015.
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