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Product Name
MMP13 antibody
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Description
MMP13 Rabbit Polyclonal antibody. Positive WB detected in MDA-MB-453s cells, A2780 cells, MCF7 cells, zebrafish tissue. Positive IF detected in MCF-7 cells. Positive IHC detected in human breast cancer tissue, human liver cancer tissue. Observed molecular weight by Western-blot: 50-52kd
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Tested applications
ELISA, IHC, WB, IF
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Species reactivity
Human,Mouse,Rat,Zebrafish; other species not tested.
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Alternative names
CLG3 antibody; Collagenase 3 antibody; Matrix metalloproteinase 13 antibody; MMP 13 antibody; MMP13 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MMP13 recombinant protein (Accession Number: NM_002427). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:112698(MMP13 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:112698(MMP13 antibody) at dilution of 1:50 (under 40x lens)
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with Catalog No:112698(MMP13 Antibody) at dilution of 1:600
Immunofluorescent analysis of MCF-7 cells using Catalog No:112698(MMP13 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
MMP-13(Matrix metalloproteinase-13), also known as collagenase-3, cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial collagenase (MMP-1). MMP-13 is found in hypertrophic and calcifying cartilage of mammalian growth plate. The bands seen with rat MMP-13 are consistent with a 59-kDa latent form and a 43-kDa active form reported for this enzyme ,along with a smaller band at approximately 30 kDa(PMID:10771523). It also can be detected 3 bands that the glycosylated proMMP-13 (70 kDa), unglycosylated proform (55 kDa), and active MMP-13 enzyme (40kDa) by western blot(PMID:18332263).
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References
- Weng T, Yi L, Huang J. Genetic inhibition of fibroblast growth factor receptor 1 in knee cartilage attenuates the degeneration of articular cartilage in adult mice. Arthritis and rheumatism. 64(12):3982-92. 2012.
- Ding Q, Zhong H, Qi Y. Anti-arthritic effects of crocin in interleukin-1β-treated articular chondrocytes and cartilage in a rabbit osteoarthritic model. Inflammation research : official journal of the European Histamine Research Society ...
- Yoshioka Y, Kozawa E, Urakawa H. Suppression of hyaluronan synthesis alleviates inflammatory responses in murine arthritis and in human rheumatoid synovial fibroblasts. Arthritis and rheumatism. 65(5):1160-70. 2013.
- Ding QH, Cheng Y, Chen WP, Zhong HM, Wang XH. Celastrol, an inhibitor of heat shock protein 90β potently suppresses the expression of matrix metalloproteinases, inducible nitric oxide synthase and cyclooxygenase-2 in primary human osteoarthritic chondrocytes. European journal of pharmacology. 708(1-3):1-7. 2013.
- Zeng L, Wang W, Rong XF. Chondroprotective effects and multi-target mechanisms of Icariin in IL-1 beta-induced human SW 1353 chondrosarcoma cells and a rat osteoarthritis model. International immunopharmacology. 18(1):175-81. 2014.
- Weng T, Xie Y, Yi L. Loss of Vhl in cartilage accelerated the progression of age-associated and surgically induced murine osteoarthritis. Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society. 22(8):1197-205. 2014.
- Ma J, Zhou D, Fan M. Keratocytes create stromal spaces to promote corneal neovascularization via MMP13 expression. Investigative ophthalmology & visual science. 55(10):6691-703. 2014.
- Wu DQ, Zhong HM, Ding QH, Ba L. Protective effects of biochanin A on articular cartilage: in vitro and in vivo studies. BMC complementary and alternative medicine. 14:444. 2014.
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