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Product Name
MMP12 antibody
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Description
MMP12 Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, A549 cells. Positive IHC detected in human lung cancer tissue, human breast cancer tissue, human lung tissue. Positive IF detected in A549 cells. Observed molecular weight by Western-blot: 54 kDa,45 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
HME antibody; Macrophage elastase antibody; Macrophage metalloelastase antibody; Matrix metalloproteinase 12 antibody; MME antibody; MMP 12 antibody; MMP12 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MMP12 recombinant protein (Accession Number: NM_002426). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:112697(MMP12 Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using Catalog No:112697(MMP12 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using Catalog No:112697(MMP12 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed A549 cells using Catalog No:112697(MMP12 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
MMP12(Matrix metalloproteinase-12) is a 54 kDa proenzyme that is processed into a 45 kDa and then a 22 kDa active form(15723202). MMP9 and MMP12 promote intimal thickening by independent cleavage of N-cadherin, which elevates vascular smooth muscle cell proliferation via beta-catenin signalling. Its overexpression in myeloid lineage cells plays a key role in modulating myelopoiesis, immune suppression, and lung tumorigenesis(PMID: 21378275).
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