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MME,CD10 antibody

Cat.#: 112693

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Product Information

  • Product Name
    MME,CD10 antibody
  • Documents
  • Description
    MME,CD10 Rabbit Polyclonal antibody. Positive IHC detected in human kidney tissue, human hepat℃irrhosis tissue, human liver cancer tissue, human renal cell carcinoma tissue. Positive FC detected in Jurkat cells. Positive WB detected in mouse kidney tissue, HEK-293 cells. Observed molecular weight by Western-blot: 90-100kd
  • Tested applications
    ELISA, WB, IHC, FC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    Atriopeptidase antibody; CALLA antibody; CD10 antibody; DKFZp686O16152 antibody; Enkephalinase antibody; EPN antibody; membrane metallo endopeptidase antibody; MME antibody; NEP antibody; Neprilysin antibody; Neutral endopeptidase antibody; Neutral endopeptidase 24.11 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of MME,CD10 recombinant protein (Accession Number: NM_001354643). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:112693(MME,CD10 antibody) at dilution of 1:500

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:112693(MME,CD10 antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:112693(MME,CD10 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:112693(MME,CD10 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:112693(MME,CD10 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:112693(MME,CD10 antibody) at dilution of 1:50 (under 40x lens)

    1X10^6 Jurkat cells were stained with 0.2ug MME,CD10 antibody (Catalog No:112693, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 Jurkat cells were stained with 0.2ug MME,CD10 antibody (Catalog No:112693, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"