MMAB antibody

Cat.#: 112690

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Product Information

  • Product Name
    MMAB antibody
  • Documents
  • Description
    MMAB Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human ovary tumor tissue. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, BxPC-3 cells, HepG2 cells, L02 cells. Observed molecular weight by Western-blot: 27 kDa
  • Tested applications
    ELISA, WB, IHC, IP, IF
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    ATR antibody; cblB antibody; MMAB antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of MMAB recombinant protein (Accession Number: NM_052845). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:50-1:500

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112690(MMAB antibody) at dilution of 1:300

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112690(MMAB antibody) at dilution of 1:300

    Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:112690(MMAB antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:112690(MMAB antibody) at dilution of 1:50 (under 10x lens)

    IP Result of anti-MMAB (IP:Catalog No:112690, 4ug; Detection:Catalog No:112690 1:500) with HeLa cells lysate 1200ug.

    IP Result of anti-MMAB (IP:Catalog No:112690, 4ug; Detection:Catalog No:112690 1:500) with HeLa cells lysate 1200ug.

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:112690(MMAB Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:112690(MMAB Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    The MMAB gene encodes cob(I)alamin adenosyltransferase, which catalyzes the final step in the synthesis of the coenzyme adenosylcobalamin (Adocbl). Adocbl is a vitamin B12-containing coenzyme for methylmalonyl-CoA mutase. The deduced 250-amino acid protein has a calculated molecular mass of 27.3 kD.
  • References
    • Jorge-Finnigan A, Aguado C, Sánchez-Alcudia R. Functional and structural analysis of five mutations identified in methylmalonic aciduria cblB type. Human mutation. 31(9):1033-42. 2010.
    • Jorge-Finnigan A, Brasil S, Underhaug J. Pharmacological chaperones as a potential therapeutic option in methylmalonic aciduria cblB type. Human molecular genetics. 22(18):3680-9. 2013.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"