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Product Name
MLXIP antibody
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Description
MLXIP Rabbit Polyclonal antibody. Positive WB detected in K-562 cells. Positive IP detected in K-562 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human skeletal muscle tissue. Observed molecular weight by Western-blot: 130 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
bHLHe36 antibody; KIAA0867 antibody; MIR antibody; MLX interacting protein antibody; MLXIP antibody; MONDOA antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MLXIP recombinant protein (Accession Number: BC039704). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:112687(MLXIP antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human skeletal muscle using Catalog No:112687(MLXIP antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human skeletal muscle using Catalog No:112687(MLXIP antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using MLXIP antibody Catalog No:112687 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-MLXIP (IP:Catalog No:112687, 4ug; Detection:Catalog No:112687 1:300) with K-562 cells lysate 3200ug.
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Background
Mlx-interacting protein(MLXIP), after forming heterodimers with MLX, can bind to and then activate transcription from CACGTG E boxes. The N terminus of MLXIP acts as a CRM1-dependent nuclear export signal and contribute to the cytoplasmic localization of the complex, and as a binding site for 13 protein. the C terminus contains a cytoplasmic localization signal and mediates protein-protein interaction of MONDOA and MLX. The complex play a role in transcriptional activation of glycolytic target genes and glucose-responsive gene regulation. This antibody could recognize isoform1(~110kd) and isoform3(~69kd) of MLXIP.
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References
- Kaadige MR, Yang J, Wilde BR, Ayer DE. MondoA-Mlx transcriptional activity is limited by mTOR-MondoA interaction. Molecular and cellular biology. 35(1):101-10. 2015.
- Shen L, O'Shea JM, Kaadige MR. Metabolic reprogramming in triple-negative breast cancer through Myc suppression of TXNIP. Proceedings of the National Academy of Sciences of the United States of America. 112(17):5425-30. 2015.
- Carroll PA, Diolaiti D, McFerrin L. Deregulated Myc requires MondoA/Mlx for metabolic reprogramming and tumorigenesis. Cancer cell. 27(2):271-85. 2015.
- Sanchez EL, Carroll PA, Thalhofer AB, Lagunoff M. Latent KSHV Infected Endothelial Cells Are Glutamine Addicted and Require Glutaminolysis for Survival. PLoS pathogens. 11(7):e1005052. 2015.
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