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Product Name
MLN51/CASC3 antibody
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Description
MLN51/CASC3 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive WB detected in A2780 cells, Jurkat cells, MCF7 cells, SKOV-3 cells. Observed molecular weight by Western-blot: 115-120 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BTZ antibody; CASC3 antibody; MLN 51 antibody; MLN51 antibody; Protein barentsz antibody; Protein CASC3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MLN51/CASC3 recombinant protein (Accession Number: XM_005257163). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:10-1:100
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Validations
A2780 cells were subjected to SDS PAGE followed by western blot with Catalog No:112684(CASC3 antibody) at dilution of 1:300
Immunofluorescent analysis of HepG2 cells, using CASC3 antibody Catalog No:112684 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
CASC3(cancer susceptibility candidate 3) is also named as MLN51 and belongs to the CASC3 family. It is induced by CSF2, and is important in the proliferation of RA fibroblast-like synoviocytes in the pathogenesis of rheumatoid arthritis (RA)(PMID:17101062). It is a nucleocytoplasmic protein containing, within its aminoterminal half, a coiled-coil domain followed by two nuclear localization signals (NLSs) responsible for nuclear localization and, in its carboxyl-terminal half, a nuclear export signal(NES) that mediates cytoplasmic retention. It possesses many potential phosphorylation sites and is found to be phosphorylated in both normal and cancer-derived cells.(PMID:17652158).
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References
- Daguenet E, Baguet A, Degot S. Perispeckles are major assembly sites for the exon junction core complex. Molecular biology of the cell. 23(9):1765-82. 2012.
- Bruno IG, Karam R, Huang L. Identification of a microRNA that activates gene expression by repressing nonsense-mediated RNA decay. Molecular cell. 42(4):500-10. 2011.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"