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MINA antibody

Cat.#: 112622

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Product Information

  • Product Name
    MINA antibody
  • Documents
  • Description
    MINA Rabbit Polyclonal antibody. Positive IHC detected in human pancreas cancer tissue. Positive WB detected in HEK-293 cells, A375 cells, mouse liver tissue. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 53 kDa
  • Tested applications
    ELISA, WB, IHC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    DKFZp762O1912 antibody; FLJ14393 antibody; MDIG antibody; MINA antibody; Mina53 antibody; MYC induced nuclear antigen antibody; NO52 antibody; Nucleolar protein 52 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of MINA recombinant protein (Accession Number: XM_005247838). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:112622(MINA antibody) at dilution of 1:500

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:112622(MINA antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:112622(MINA antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:112622(MINA antibody) at dilution of 1:50 (under 10x lens)

    IP Result of anti-MINA (IP:Catalog No:112622, 3ug; Detection:Catalog No:112622 1:500) with HEK-293 cells lysate 2120ug.

    IP Result of anti-MINA (IP:Catalog No:112622, 3ug; Detection:Catalog No:112622 1:500) with HEK-293 cells lysate 2120ug.

  • Background
    MINA, also named as MDIG, MINA53 and NO52, belongs to the MINA53/NO66 family. It is involved in cellular proliferation. MINA may play an important role in cell growth and survival. It is involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles. The transcriptional repressor MINA has been shown to repress Il4 gene expression by directly binding to its promoter, thereby, controlling TH2 bias.(PMID:21499227)
  • References
    • Kashiwada M, Cassel SL, Colgan JD, Rothman PB. NFIL3/E4BP4 controls type 2 T helper cell cytokine expression. The EMBO journal. 30(10):2071-82. 2011.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"