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Product Name
MIF antibody
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Description
MIF Rabbit Polyclonal antibody. Positive IHC detected in human lung tissue, human testis tissue. Positive WB detected in Y79 cells, Jurkat cells, rat spleen tissue, U-937 cells. Positive IP detected in mouse spleen tissue. Observed molecular weight by Western-blot: 12 kDa
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human, Rat; other species not tested.
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Alternative names
GIF antibody; GLIF antibody; L dopachrome isomerase antibody; L dopachrome tautomerase antibody; MIF antibody; MMIF antibody; Phenylpyruvate tautomerase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MIF recombinant protein (Accession Number: NM_002415). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
Y79 cells were subjected to SDS PAGE followed by western blot with Catalog No:112619(MIF antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human lung using Catalog No:112619(MIF antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung using Catalog No:112619(MIF antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-MIF (IP:Catalog No:112619, 4ug; Detection:Catalog No:112619 1:1000) with mouse spleen tissue lysate 4000ug.
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Background
MIF represents macrophage migration inhibitory factor (glycosylation-inhibiting factor)
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References
- Li S, Li X, Wang Y, Yang J, Chen Z, Shan S. Global secretome characterization of A549 human alveolar epithelial carcinoma cells during Mycoplasma pneumoniae infection. BMC microbiology. 14:27. 2014.
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