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Product Name
MGMT antibody
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Description
MGMT Rabbit Polyclonal antibody. Positive IP detected in Jurkat cells. Positive WB detected in Jurkat cells, MCF7 cells. Positive IHC detected in human kidney tissue, human placenta tissue, human spleen tissue, human testis tissue. Observed molecular weight by Western-blot: 22 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
MGMT antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MGMT recombinant protein (Accession Number: XM_005252682). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:112603(MGMT antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human kidney using Catalog No:112603(MGMT antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:112603(MGMT antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-MGMT (IP:Catalog No:112603, 4ug; Detection:Catalog No:112603 1:500) with Jurkat cells lysate 3200ug.
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Background
MGMT is the primary vehicle for cellular removal of alkyl lesions from the O-6 position of guanine and the O-4 position of thymine. While key to the maintenance of genomic integrity, MGMT also removes damage induced by alkylating chemotherapies, inhibiting the efficacy of cancer treatment [PMID:23065697].MGMT is the primary mechanism for the removal of alkylation damage from the O-6 position of guanine [PMID: 17482892]. The O-6 position of guanine is one of several positions in DNA bases to which alkyl groups are attached in SN1 alkylation reactions, and this repair has been well-characterized in mammalian cells and via MGMT homologs in bacteria and Archaea.[PMID: 10767620]
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References
- Duan H, He Z, Ma J. Global and MGMT promoter hypomethylation independently associated with genomic instability of lymphocytes in subjects exposed to high-dose polycyclic aromatic hydrocarbon. Archives of toxicology. 87(11):2013-22. 2013.
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