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Product Name
MFN1 antibody
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Description
MFN1 Rabbit Polyclonal antibody. Positive IHC detected in human lung cancer tissue. Positive WB detected in human kidney tissue. Observed molecular weight by Western-blot: 86kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp762F247 antibody; FLJ20693 antibody; Fzo homolog antibody; hfzo1 antibody; hfzo2 antibody; MFN1 antibody; mitofusin 1 antibody; Transmembrane GTPase MFN1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MFN1 recombinant protein (Accession Number: XM_005247596). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
human kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:112529(MFN1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:112529(MFN1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:112529(MFN1 antibody) at dilution of 1:100 (under 40x lens)
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Background
Mitofusin-1 (MFN1) is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). Mitofusins are large predicted GTPases located in outer mitochondrial membrane. They are essential for outer membrane fusion by interacting with each other to facilitate mitochondrial targeting. The mitofusins are the first known protein mediator of mitochondrial fusion, and mediate developmentally regulated post-meiotic fusion of mitochondria.Mfn1 is required on adjacent mitochondria to mediate fusion via interactions of a heptad repeat region that mediates oligomerization of the protein(PMID:16892085).
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References
- Park YY, Nguyen OT, Kang H, Cho H. MARCH5-mediated quality control on acetylated Mfn1 facilitates mitochondrial homeostasis and cell survival. Cell death & disease. 5:e1172. 2014.
- Leduc-Gaudet JP, Picard M, St-Jean Pelletier F. Mitochondrial morphology is altered in atrophied skeletal muscle of aged mice. Oncotarget. 6(20):17923-37. 2015.
- Li H, Ruan Y, Zhang K. Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization. Cell death and differentiation. 23(3):380-92. 2016.
- Sharoar MG, Shi Q, Ge Y. Dysfunctional tubular endoplasmic reticulum constitutes a pathological feature of Alzheimer's disease. Molecular psychiatry. 2015.
- Park YY, Cho H. Mitofusin 1 is degraded at G2/M phase through ubiquitylation by MARCH5. Cell division. 7(1):25. 2012.
- Hoshino A, Mita Y, Okawa Y. Cytosolic p53 inhibits Parkin-mediated mitophagy and promotes mitochondrial dysfunction in the mouse heart. Nature communications. 4:2308. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"