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METTL3 antibody

Cat.#: 112518

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Product Information

  • Product Name
    METTL3 antibody
  • Documents
  • Description
    METTL3 Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells, HeLa cells, HepG2 cells. Observed molecular weight by Western-blot: 70 kDa
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    IME4 antibody; M6A antibody; methyltransferase like 3 antibody; METTL3 antibody; MT A70 antibody; MTA70 antibody; Spo8 antibody
  • Immunogen
    Human METTL3 Recombinant protein (His tag)
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of METTL3 recombinant protein (Accession Number: NM_019852). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

  • Validations

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:112518(METTL3 antibody) at dilution of 1:400

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:112518(METTL3 antibody) at dilution of 1:400

    IP Result of anti-METTL3 (IP:Catalog No:112518, 4ug; Detection:Catalog No:112518 1:1000) with HEK-293 cells lysate 4500ug.

    IP Result of anti-METTL3 (IP:Catalog No:112518, 4ug; Detection:Catalog No:112518 1:1000) with HEK-293 cells lysate 4500ug.

  • Background
    METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs.
  • References
    • Fustin JM, Doi M, Yamaguchi Y. RNA-methylation-dependent RNA processing controls the speed of the circadian clock. Cell. 155(4):793-806. 2013.
    • Wang X, Lu Z, Gomez A. N6-methyladenosine-dependent regulation of messenger RNA stability. Nature. 505(7481):117-20. 2014.
    • Liu J, Yue Y, Han D. A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation. Nature chemical biology. 10(2):93-5. 2014.
    • Zhao X, Yang Y, Sun BF. FTO-dependent demethylation of N6-methyladenosine regulates mRNA splicing and is required for adipogenesis. Cell research. 24(12):1403-19. 2014.
    • Batista PJ, Molinie B, Wang J. m(6)A RNA modification controls cell fate transition in mammalian embryonic stem cells. Cell stem cell. 15(6):707-19. 2014.
    • Chen T, Hao YJ, Zhang Y. m(6)A RNA methylation is regulated by microRNAs and promotes reprogramming to pluripotency. Cell stem cell. 16(3):289-301. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"