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Product Name
MEPCE antibody
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Description
MEPCE Rabbit Polyclonal antibody. Positive WB detected in K-562 cells. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 74 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat,Zebrafish; other species not tested.
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Alternative names
Bin3 homolog antibody; FLJ20257 antibody; MEPCE antibody; methylphosphate capping enzyme antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MEPCE recombinant protein (Accession Number: NM_019606). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:112657(MEPCE antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:112657(MEPCE antibody) at dilution of 1:50 (under 10x lens)
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Background
MEPCE is a Component of the 7SK snRNP complex, and a S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, which leads to its stabilization.
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References
- Barboric M, Lenasi T, Chen H, Johansen EB, Guo S, Peterlin BM. 7SK snRNP/P-TEFb couples transcription elongation with alternative splicing and is essential for vertebrate development. Proceedings of the National Academy of Sciences of the United States of America. 106(19):7798-803. 2009.
- Eilebrecht S, Brysbaert G, Wegert T, Urlaub H, Benecke BJ, Benecke A. 7SK small nuclear RNA directly affects HMGA1 function in transcription regulation. Nucleic acids research. 39(6):2057-72. 2011.
- Eilebrecht S, Benecke BJ, Benecke A. 7SK snRNA-mediated, gene-specific cooperativity of HMGA1 and P-TEFb. RNA biology. 8(6):1084-93. 2012.
- Elagib KE, Rubinstein JD, Delehanty LL. Calpain 2 activation of P-TEFb drives megakaryocyte morphogenesis and is disrupted by leukemogenic GATA1 mutation. Developmental cell. 27(6):607-20. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"