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Product Name
MEK2 antibody
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Description
MEK2 Rabbit Polyclonal antibody. Positive IP detected in HL-60 cells. Positive WB detected in HL-60 cells, human brain tissue, Jurkat cells, SH-SY5Y cells. Positive IF detected in SH-SY5Y cells. Observed molecular weight by Western-blot: 44-47 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ERK activator kinase 2 antibody; FLJ26075 antibody; MAP kinase kinase 2 antibody; MAP2K2 antibody; MAPK/ERK kinase 2 antibody; MAPKK 2 antibody; MAPKK2 antibody; MEK 2 antibody; MEK2 antibody; MKK2 antibody; PRKMK2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MEK2 recombinant protein (Accession Number: NM_030662). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IF: 1:10-1:100
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Validations
HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:112643(MAP2K2 antibody) at dilution of 1:1000
IP Result of anti-MAP2K2 (IP:Catalog No:112643, 4ug; Detection:Catalog No:112643 1:1000) with HL-60 cells lysate 1280ug.
Immunofluorescent analysis of SH-SY5Y cells using Catalog No:112643(MAP2K2 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and Belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway. 11049-1-AP can recognize both the MEK2 and MEK1.
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References
- Wang Y, Luo H, Li Y, Chen T, Wu S, Yang L. hsa-miR-96 up-regulates MAP4K1 and IRS1 and may function as a promising diagnostic marker in human bladder urothelial carcinomas. Molecular medicine reports. 5(1):260-5. 2012.
- Li XL, Chen XQ, Zhang MN. SOX9 was involved in TKIs resistance in renal cell carcinoma via Raf/MEK/ERK signaling pathway. International journal of clinical and experimental pathology. 8(4):3871-81. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"