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Product Name
MED20 antibody
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Description
MED20 Rabbit Polyclonal antibody. Positive WB detected in K-562 cells, HeLa cells. Observed molecular weight by Western-blot: 28kd
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Tested applications
ELISA, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp586D2223 antibody; hTRFP antibody; MED20 antibody; mediator complex subunit 20 antibody; PRO0213 antibody; TRF proximal protein homolog antibody; TRFP antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MED20 recombinant protein (Accession Number: NM_004275). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:112587(MED20 antibody) at dilution of 1:500
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Background
MED20(Mediator of RNA polymerase II transcription subunit 20) is also named as TRFP and belongs to the Mediator complex subunit 20 family. The deduced 209-amino acid protein shares 44% amino identity with Drosophila Trfp. TRFP had an apparent molecular mass of 28 kD by SDS-PAGE. It is identified TRFP as a component of an RNA polymerase II-SRB complex. The TRFP-containing complex could substitute for core RNA polymerase II in driving basal transcription from a minimal promoter.
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References
- Vijayalingam S, Chinnadurai G. Adenovirus L-E1A activates transcription through mediator complex-dependent recruitment of the super elongation complex. Journal of virology. 87(6):3425-34. 2013.
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