Product Name

ME1 antibody

Documents

Description

ME1 Rabbit Polyclonal antibody. Positive IHC detected in human liver tissue, human placenta tissue. Positive WB detected in mouse liver tissue, mouse placenta tissue. Positive IP detected in mouse liver tissue. Observed molecular weight by Western-blot: 55-64kd

Tested applications

ELISA, WB, IHC, IP

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

HUMNDME antibody; Malic enzyme 1 antibody; ME1 antibody; MES antibody; NADP dependent malic enzyme antibody; NADP ME antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of ME1 recombinant protein (Accession Number: NM_002395). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:200-1:2000

IHC: 1:20-1:200

Validations

mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:112574(ME1 antibody) at dilution of 1:2000

Immunohistochemical of paraffin-embedded human liver using Catalog No:112574(ME1 antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human liver using Catalog No:112574(ME1 antibody) at dilution of 1:100 (under 40x lens)

IP Result of anti-ME1 (IP:Catalog No:112574, 4ug; Detection:Catalog No:112574 1:500) with mouse liver tissue lysate 4000ug.

Background

ME1(NADP-dependent malic enzyme) belongs to the malic enzymes family. This malic enzyme catalyzes the reversible oxidative decarboxylation of malate and is a link between the glycolytic pathway and the citric acid cycle. The reaction is L-malate plus NADP(+) to form pyruvate, CO(2), and NADPH. The predicted protein contains 572 amino acids and has a calculated molecular mass of 64.1 kDa.

References

Peinado JR, Quirós PM, Pulido MR. Proteomic profiling of adipose tissue from Zmpste24-/- mice, a model of lipodystrophy and premature aging, reveals major changes in mitochondrial function and vimentin processing. Molecular & cellular proteomics : MCP. 10(11):M111.008094. 2011.
Al-Dwairi A, Pabona JM, Simmen RC, Simmen FA. Cytosolic malic enzyme 1 (ME1) mediates high fat diet-induced adiposity, endocrine profile, and gastrointestinal tract proliferation-associated biomarkers in male mice. PloS one. 7(10):e46716. 2012.
Mitsuishi Y, Taguchi K, Kawatani Y. Nrf2 redirects glucose and glutamine into anabolic pathways in metabolic reprogramming. Cancer cell. 22(1):66-79. 2012.
Al-Dwairi A, Brown AR, Pabona JM. Enhanced gastrointestinal expression of cytosolic malic enzyme (ME1) induces intestinal and liver lipogenic gene expression and intestinal cell proliferation in mice. PloS one. 9(11):e113058. 2014.
Sun L, Song L, Wan Q. cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions. Cell research. 25(4):429-44. 2015.

ME1 antibody

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