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Product Name
MCM5 antibody
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Description
MCM5 Rabbit Polyclonal antibody. Positive IHC detected in human lymphoma tissue. Positive IP detected in Jurkat cells. Positive WB detected in Jurkat cells, A375 cells, HeLa cells, mouse thymus tissue. Observed molecular weight by Western-blot: 82kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CDC46 antibody; CDC46 homolog antibody; MCM5 antibody; P1 CDC46 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MCM5 recombinant protein (Accession Number: NM_006739). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:112555(MCM5 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:112555(MCM5 antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-MCM5 (IP:Catalog No:112555, 4ug; Detection:Catalog No:112555 1:500) with Jurkat cells lysate 2400ug.
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Background
Minichromosome maintenance, S. Cerevisiae, homolog of 5(MCM5), one component of the complex of MCM2-7, which is the putative replicative essential for once per cell cycle DNA replication initiation and elongation in eukaryotic cells. The MCM complex form a hexameric ring through the interaction surfaces of two neighboring subunits.
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References
- Liu YZ, Jiang YY, Wang BS. A panel of protein markers for the early detection of lung cancer with bronchial brushing specimens. Cancer cytopathology. 122(11):833-41. 2014.
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