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Product Name
MBIP antibody
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Description
MBIP Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue, human lung cancer tissue. Positive IF detected in HepG2 cells. Positive WB detected in HEK-293 cells, HepG2 cells. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 45 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
MBIP antibody; MUK binding inhibitory protein antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MBIP recombinant protein (Accession Number: NM_016586). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:112506(MBIP antibody) at dilution of 1:300
IP Result of anti-MBIP (IP:Catalog No:112506, 4ug; Detection:Catalog No:112506 1:300) with HEK-293 cells lysate 2800ug.
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:112506(MBIP Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:112506(MBIP Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:112506(MBIP Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
MBIP (MAP3K12 binding inhibitory protein 1), also known as MAPK upstream kinase (MUK)-binding inhibitory protein, is a 344 amino acid nuclear and cytoplasmic protein that assists in the activation of the JNK pathway by inhibiting ZPK activity. Ubiquitously expressed, MBIP is found at high levels in lung and heart and is one component of the ADA2A-containing complex (ATAC) in conjunction with ADA3, GCN5, CDC101, TADA2L, WDR5, YEATS2, DR1 and CRP2BP.
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References
- Weir BA, Woo MS, Getz G. Characterizing the cancer genome in lung adenocarcinoma. Nature. 450(7171):893-8. 2007.
- Guelman S, Kozuka K, Mao Y. The double-histone-acetyltransferase complex ATAC is essential for mammalian development. Molecular and cellular biology. 29(5):1176-88. 2009.
- Sela D, Chen L, Martin-Brown S. Endoplasmic reticulum stress-responsive transcription factor ATF6α directs recruitment of the Mediator of RNA polymerase II transcription and multiple histone acetyltransferase complexes. The Journal of biological chemistry. 287(27):23035-45. 2012.
- Wang YL, Faiola F, Xu M, Pan S, Martinez E. Human ATAC Is a GCN5/PCAF-containing acetylase complex with a novel NC2-like histone fold module that interacts with the TATA-binding protein. The Journal of biological chemistry. 283(49):33808-15. 2008.
- Jin Q, Yu LR, Wang L. Distinct roles of GCN5/PCAF-mediated H3K9ac and CBP/p300-mediated H3K18/27ac in nuclear receptor transactivation. The EMBO journal. 30(2):249-62. 2011.
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