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Product Name
MBIP antibody
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Description
MBIP Mouse Monoclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in 293 cells, HepG2 cells, human heart tissue. Positive IHC detected in human heart, human heart tissue. Observed molecular weight by Western-blot: 39 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
MBIP antibody; MUK binding inhibitory protein antibody
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of MBIP recombinant protein (Accession Number: NM_016586). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:107416(MBIP antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human heart slide using Catalog No:107416(MBIP Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human heart slide using Catalog No:107416(MBIP Antibody) at dilution of 1:50
IP Result of anti-MBIP (IP:Catalog No:107416, 3ug; Detection:Catalog No:107416 1:300) with HEK-293 cells lysate 1800ug.
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Background
MBIP (MAP3K12 binding inhibitory protein 1), also known as MAPK upstream kinase (MUK)-binding inhibitory protein, is a 344 amino acid nuclear and cytoplasmic protein that assists in the activation of the JNK pathway by inhibiting ZPK activity. Ubiquitously expressed, MBIP is found at high levels in lung and heart and is one component of the ADA2A-containing complex (ATAC) in conjunction with ADA3, GCN5, CDC101, TADA2L, WDR5, YEATS2, DR1 and CRP2BP.
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