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Product Name
MARK2 antibody
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Description
MARK2 Rabbit Polyclonal antibody. Positive IHC detected in human brain tissue. Positive IP detected in rat brain tissue. Positive WB detected in mouse brain tissue. Observed molecular weight by Western-blot: 78 kDa,80 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ELKL motif kinase 1 antibody; EMK 1 antibody; EMK1 antibody; MARK2 antibody; PAR 1 antibody; PAR1 homolog antibody; Par1b antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MARK2 recombinant protein (Accession Number: BC008771). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:112479(MARK2 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human brain using Catalog No:112479(MARK2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:112479(MARK2 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-MARK2 (IP:Catalog No:112479, 4ug; Detection:Catalog No:112479 1:1000) with rat brain tissue lysate 4000ug.
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Background
MARK2 is also named as EMK1(ELKL motif kinase 1), Par1b and belongs to the CAMK Ser/Thr protein kinase family. Par1b/MARK2 plays a critical role in neuronal polarity in the process of axon specification from multiple candidate neurites using primary cultures of mammalian hippocampal neurons(PMID:20194617). It has 16 isoforms produced by alternative promoter usage and alternative splicing and can exsit as a dimer(PMID:16803889).
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References
- Goodwin JM, Svensson RU, Lou HJ, Winslow MM, Turk BE, Shaw RJ. An AMPK-independent signaling pathway downstream of the LKB1 tumor suppressor controls Snail1 and metastatic potential. Molecular cell. 55(3):436-50. 2014.
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