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Product Name
MARCKS antibody
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Description
MARCKS Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells, Hela cells. Positive IHC detected in human heart tissue, human placenta tissue. Positive IP detected in A431 cells. Positive WB detected in HEK-293 cells, A431 cells, mouse brain tissue. Observed molecular weight by Western-blot: 80 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
80K L antibody; 80K L protein antibody; FLJ14368 antibody; FLJ90045 antibody; MACS antibody; MARCKS antibody; PKCSL antibody; PRKCSL antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_002356). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:112447(MARCKS antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human heart using Catalog No:112447(MARCKS antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:112447(MARCKS antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using MARCKS antibody Catalog No:112447 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-MARCKS (IP:Catalog No:112447, 4ug; Detection:Catalog No:112447 1:300) with A431 cells lysate 1200ug.
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Background
MARCKS, also named as MACS, PRKCSL and 80K-L protein, belongs to the MARCKS family. MARCKS is the most prominent cellular substrate for protein kinase C. It binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
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