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Product Name
MARCH8 antibody
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Description
MARCH8 Rabbit Polyclonal antibody. Positive WB detected in mouse testis tissue. Positive IP detected in mouse lung tissue. Observed molecular weight by Western-blot: 30-33kd
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Tested applications
ELISA, WB, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
c MIR antibody; MARCH VIII antibody; MARCH8 antibody; MIR antibody; RING finger protein 178 antibody; RNF178 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MARCH8 recombinant protein (Accession Number: XM_047424767). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
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Validations
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:112446(MARCH8 antibody) at dilution of 1:800
IP Result of anti-MARCH8 (IP:Catalog No:112446, 3ug; Detection:Catalog No:112446 1:400) with mouse lung tissue lysate 4000ug.
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Background
MARCH8(Membrane-associated RING finger protein 8) is also named as MIR, RNF178 and belongs to the RING zinc finger protein family. It functions as an E3 ubiquitin ligase for immune recognition-related molecules (e.g. major histocompatibility complex class I, B7-2, and ICAM-1). It plays a key role in controlling MHC class II surface expression by regulated ubiquitination of a lysine residue in the beta-chain(PMID:22761441).
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References
- Chen R, Li M, Zhang Y, Zhou Q, Shu HB. The E3 ubiquitin ligase MARCH8 negatively regulates IL-1β-induced NF-κB activation by targeting the IL1RAP coreceptor for ubiquitination and degradation. Proceedings of the National Academy of Sciences of the United States of America. 109(35):14128-33. 2012.
- Kim MH, Rebbert ML, Ro H, Won M, Dawid IB. Cell adhesion in zebrafish embryos is modulated by March 8. PloS one. 9(4):e94873. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"