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Product Name
MAGED1 antibody
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Description
MAGED1 Rabbit Polyclonal antibody. Positive IHC detected in human placenta tissue, human skin tissue. Positive IF detected in A549 cells. Positive FC detected in HeLa cells. Positive IP detected in A549 cells. Positive WB detected in A549 cells, HEK-293 cells, mouse placenta tissue. Observed molecular weight by Western-blot: 100 kDa
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Tested applications
ELISA, IHC, IF, IP, WB, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DLXIN 1 antibody; MAGE D1 antigen antibody; MAGE tumor antigen CCF antibody; MAGED1 antibody; melanoma antigen family D antibody; Melanoma assOCiated antigen D1 antibody; NRAGE antibody; PP2250 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MAGED1 recombinant protein (Accession Number: NM_001005333). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:112390(MAGED1 Antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human placenta using Catalog No:112390(MAGED1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human placenta using Catalog No:112390(MAGED1 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-MAGED1 (IP:Catalog No:112390, 4ug; Detection:Catalog No:112390 1:1000) with A549 cells lysate 400ug.
Immunofluorescent analysis of A549 cells using Catalog No:112390(MAGED1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 HeLa cells were stained with .2ug MAGED1 antibody (Catalog No:112390, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
MAGED1 (also known as NRAGE or Dlxin-1) is a member of the type II melanoma antigen (MAGE) family of protein. MAGED1 can interact with the nerve growth factor receptor p75NTR, antagonize the association of p75NTR with TrkA, inhibit cell cycle progression, and facilitate p75NTR-mediated apoptosis (PMID: 10985348). It has been reported that MAGED1 binds Dlx5 and regulates its transcriptional function. In vivo, MAGED1 binds not only Dlx5 but also Dlx7 and Msx2 and forms homomultimers (PMID: 11084035). MAGED1 knockout mice show depressive behavior and impairments of circadian rhythm (PMID: 20300063; 23314527). Three transcript variants encoding two different isoforms (86kDa and 92 kDa) have been found for the gene of MAGED1.
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References
- Zhou H, Zhang G, Xue X. Identification of novel NRAGE involved in the radioresistance of esophageal cancer cells. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 2016.
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