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Product Name
MAD2L1BP antibody
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Description
MAD2L1BP Rabbit Polyclonal antibody. Positive IF detected in A431 cells. Positive IHC detected in human skin tissue, human colon tissue. Positive WB detected in HeLa cells, K-562 cells. Observed molecular weight by Western-blot: 34kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Caught by MAD2 protein antibody; CMT2 antibody; KIAA0110 antibody; MAD2L1 binding protein antibody; MAD2L1BP antibody; RP1 261G23.6 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MAD2L1BP recombinant protein (Accession Number: NM_014628). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112368(MAD2L1BP antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human skin using Catalog No:112368(MAD2L1BP antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human skin using Catalog No:112368(MAD2L1BP antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of A431 cells using Catalog No:112368(MAD2L1BP Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
MAD2L1BP, also named as CMT2 and KIAA0110, belongs to the MAD2L1BP family. It may function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex. MAD2L1BP interacts with the spindle checkpoint and coordinates cell cycle events in late mitosis. MAD2L1BP may play an active role to remove MAD2 from p55CDC and tether MAD2 for silencing the checkpoint.
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