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Product Name
LPCAT1 antibody
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Description
LPCAT1 Mouse Monoclonal antibody. Positive WB detected in MCF7 cells. Positive IF detected in Hela. Observed molecular weight by Western-blot: 55kD
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Tested applications
ELISA, WB, IF
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Species reactivity
human; other species not tested.
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Alternative names
LPCAT1 antibody; lpcat antibody; LPC acyltransferase 1 antibody; FLJ41609 antibody; FLJ12443 antibody
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of LPCAT1 recombinant protein (Accession Number: BC020166). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:20-1:200
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:107408(LPCAT1 antibody) at dilution of 1:500
Immunofluorescent analysis of Hela cells, using LPCAT1 antibody Catalog No: at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
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Background
LPCAT1, also named as AYTL2, PFAAP3 and LysoPAFAT, belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. It is a key enzyme for remodeling phospholipids, including phosphatidylcholine. The expression level of LPCAT1 is able to differentiate prostate cancer from noncancerous prostatic changes, and correlates to the tumor grade of prostate cancer. LPCAT1 possesses both acyltransferase and acetyltransferase activities. It mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"