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Product Name
LMOD1 antibody
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Description
LMOD1 Rabbit Polyclonal antibody. Positive IHC detected in human ovary tumor tissue. Positive IF detected in A375 cells. Positive IP detected in mouse skeletal muscle tissue. Positive WB detected in human skeletal muscle tissue, HeLa cells, mouse bladder tissue, mouse skeletal muscle tissue, mouse uterus tissue. Observed molecular weight by Western-blot: 85kd or 64kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
64 kDa autoantigen 1D antibody; 64 kDa autoantigen 1D3 antibody; 64 kDa autoantigen D1 antibody; 64kD antibody; FLJ55689 antibody; Leiomodin 1 antibody; leiomodin 1 (smooth muscle) antibody; Leiomodin antibody; muscle form antibody; LMOD1 antibody; SM LMOD antibody; Smooth muscle leiomodin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of LMOD1 recombinant protein (Accession Number: BC001755). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:112284(LMOD1 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:112284(LMOD1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:112284(LMOD1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of A375 cells, using LMOD1 antibody Catalog No:112284 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-LMOD1 (IP:Catalog No:112284, 4ug; Detection:Catalog No:112284 1:700) with mouse skeletal muscle tissue lysate 3600ug.
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Background
LMOD1 was initially identified as a 64-kDa autoantigen in an expression screen with sera from patients with Hashimoto’s thyroiditis. Recently immunohistochemistry and immunoblotting of LMOD1 demonstrated its highly restrictive pattern of expression in SMC lineages,like adult aorta, and bladder. LMOD1 is also shown to exhibit an embryonic and adult expression pattern. These data identify LMOD1 as a new SMC-restricted gene associated with the SMC differentiated phenotype.
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References
- Nanda V, Miano JM. Leiomodin 1, a new serum response factor-dependent target gene expressed preferentially in differentiated smooth muscle cells. The Journal of biological chemistry. 287(4):2459-67. 2012.
- Bell RD, Long X, Lin M. Identification and initial functional characterization of a human vascular cell-enriched long noncoding RNA. Arteriosclerosis, thrombosis, and vascular biology. 34(6):1249-59. 2014.
- Scheffer DI, Shen J, Corey DP, Chen ZY. Gene Expression by Mouse Inner Ear Hair Cells during Development. The Journal of neuroscience : the official journal of the Society for Neuroscience. 35(16):6366-80. 2015.
- Han Y, Slivano OJ, Christie CK, Cheng AW, Miano JM. CRISPR-Cas9 genome editing of a single regulatory element nearly abolishes target gene expression in mice--brief report. Arteriosclerosis, thrombosis, and vascular biology. 35(2):312-5. 2015.
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