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LMNB2 antibody

Cat.#: 112282

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Product Information

  • Product Name
    LMNB2 antibody
  • Documents
  • Description
    LMNB2 Rabbit Polyclonal antibody. Positive FC detected in HEK-293T cells. Positive IF detected in HepG2 cells, SH-SY5Y cells. Positive IHC detected in human colon cancer tissue. Positive IP detected in Jurkat cells. Positive WB detected in Jurkat cells, BxPC-3 cells, HEK-293 cells, HeLa cells, HL-60 cells, MCF7 cells, mouse brain tissue. Observed molecular weight by Western-blot: 66-68kd
  • Tested applications
    ELISA, WB, IHC, IF, FC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    LAMB2 antibody; lamin B2 antibody; LMN2 antibody; LMNB2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of LMNB2 recombinant protein (Accession Number: BC006551). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:1000-1:10000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:112282(LMNB2 antibody) at dilution of 1:2000

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:112282(LMNB2 antibody) at dilution of 1:2000

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:112282(LMNB2 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:112282(LMNB2 antibody) at dilution of 1:100 (under 10x lens)

    Immunofluorescent analysis of HepG2 cells, using LMNB2 antibody Catalog No:112282 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of HepG2 cells, using LMNB2 antibody Catalog No:112282 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    IP Result of anti-LMNB2 (IP:Catalog No:112282, 3ug; Detection:Catalog No:112282 1:500) with Jurkat cells lysate 2400ug.

    IP Result of anti-LMNB2 (IP:Catalog No:112282, 3ug; Detection:Catalog No:112282 1:500) with Jurkat cells lysate 2400ug.

    1X10^6 HEK-293T cells were stained with 0.2ug LMNB2 antibody (Catalog No:112282, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 HEK-293T cells were stained with 0.2ug LMNB2 antibody (Catalog No:112282, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    LMNB2 represents lamin B2
  • References
    • Kuga T, Nie H, Kazami T. Lamin B2 prevents chromosome instability by ensuring proper mitotic chromosome segregation. Oncogenesis. 3:e94. 2014.
    • Kuga T, Nozaki N, Matsushita K, Nomura F, Tomonaga T. Phosphorylation statuses at different residues of lamin B2, B1, and A/C dynamically and independently change throughout the cell cycle. Experimental cell research. 316(14):2301-12. 2010.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"