LIN28B antibody

Cat.#: 112233

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Product Information

  • Product Name
    LIN28B antibody
  • Documents
  • Description
    LIN28B Rabbit Polyclonal antibody. Positive WB detected in mouse embryo tissue, HEK-293 cells. Positive IP detected in HEK-293 cells. Positive IF detected in HEK-293 cells. Observed molecular weight by Western-blot: 27 kDa
  • Tested applications
    ELISA, WB, IP, IF
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    CSDD2 antibody; FLJ16517 antibody; lin 28 homolog B (C. elegans) antibody; Lin 28B antibody; LIN28B antibody; Protein lin 28 homolog B antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of LIN28B recombinant protein (Accession Number: NM_001004317). Purification method: Antigen Affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:1000

    IP: 1:200-1:2000

    IF: 1:50-1:500

  • Validations

    mouse embryo tissue were subjected to SDS PAGE followed by western blot with Catalog No:112233(LIN28B Antibody) at dilution of 1:300

    mouse embryo tissue were subjected to SDS PAGE followed by western blot with Catalog No:112233(LIN28B Antibody) at dilution of 1:300

    IP Result of anti-LIN28B (IP:Catalog No:112233, 4ug; Detection:Catalog No:112233 1:500) with HEK-293 cells lysate 1480ug.

    IP Result of anti-LIN28B (IP:Catalog No:112233, 4ug; Detection:Catalog No:112233 1:500) with HEK-293 cells lysate 1480ug.

    Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using Catalog No:112233(LIN28B Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using Catalog No:112233(LIN28B Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    LIN28B represents lin-28 homolog B (C. elegans)
  • References
    • Hu Q, Peng J, Liu W. Lin28B is a novel prognostic marker in gastric adenocarcinoma. International journal of clinical and experimental pathology. 7(8):5083-92. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"