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Product Name
LEPREL1 antibody
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Description
LEPREL1 Rabbit Polyclonal antibody. Positive IP detected in mouse kidney tissue. Positive WB detected in mouse kidney tissue, HEK-293 cells, human placenta tissue, L02 cells, mouse placenta tissue. Positive IHC detected in human liver tissue, human brain tissue, human kidney tissue, human lung tissue, human placenta tissue, human skin tissue, human testis tissue. Observed molecular weight by Western-blot: 80 kDa
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Tested applications
ELISA, IHC, IP, WB
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
FLJ10718 antibody; leprecan like 1 antibody; Leprecan like protein 1 antibody; LEPREL1 antibody; MLAT4 antibody; P3H2 antibody; Prolyl 3 hydroxylase 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of LEPREL1 recombinant protein (Accession Number: NM_001134418). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:112198(LEPREL1 antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human liver using Catalog No:112198(LEPREL1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:112198(LEPREL1 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-LEPREL1 (IP:Catalog No:112198, 4ug; Detection:Catalog No:112198 1:600) with mouse kidney tissue lysate 4000ug.
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Background
LEPREL1 represents leprecan-like 1
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References
- Hudson DM, Joeng KS, Werther R. Post-translationally abnormal collagens of prolyl 3-hydroxylase-2 null mice offer a pathobiological mechanism for the high myopia linked to human LEPREL1 mutations. The Journal of biological chemistry. 290(13):8613-22. 2015.
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