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Product Name
LDHC-Specific antibody
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Description
LDHC-Specific Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive IHC detected in human testis tissue, human heart tissue. Positive IP detected in mouse testis tissue. Positive WB detected in human testis tissue, A549 cells. Observed molecular weight by Western-blot: 36 kDa
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Tested applications
ELISA, IHC, IF, WB, IP
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Species reactivity
Human; other species not tested.
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Alternative names
Cancer/testis antigen 32 antibody; CT32 antibody; lactate dehydrogenase C antibody; LDH C antibody; LDH testis subunit antibody; LDH X antibody; LDH3 antibody; LDHC antibody; LDHX antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_002301). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:112186(LDHC antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human testis using Catalog No:112186(LDHC antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:112186(LDHC antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using LDHC antibody Catalog No:112186 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-LDHC (IP:Catalog No:112186, 4ug; Detection:Catalog No:112186 1:500) with mouse testis tissue lysate 2400ug.
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Background
LDHC, also named as CT32 and LDH-X, belongs to the LDH/MDH superfamily and LDH family. LDHC is a possible role in sperm motility. LDHC catalyzes the reaction: (S)-lactate + NAD+ = pyruvate + NADH. LDHC is a testis-specific isoform. Abnormal LDHC expression is associated with several forms of cancer. The antibody has no cross reaction with LDHA and LDHB.
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