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Product Name
LDHB-Specific antibody
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Description
LDHB-Specific Rabbit Polyclonal antibody. Positive WB detected in HeLa cells. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 36kd
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
lactate dehydrogenase B antibody; LDH B antibody; LDH H antibody; LDH heart subunit antibody; LDHB antibody; TRG 5 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_002300). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112185(LDHB antibody) at dilution of 1:300
Immunofluorescent analysis of Hela cells, using LDHB antibody 19988-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
LDHB, also named as NY-REN-46 and LDH-H, belongs to the LDH/MDH superfamily and LDH family. LDHB catalyzes the reaction: (S)-lactate + NAD+ = pyruvate + NADH. It is an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway.It is glycolytic enzymes and it is positively regulated by mTOR.Defects in LDHB are a cause of hereditary LDHB deficiency. The antibody has no cross reaction with LDHA and LDHC.
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