LCK antibody

Cat.#: 112172

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Product Information

  • Product Name
    LCK antibody
  • Documents
  • Description
    LCK Rabbit Polyclonal antibody. Positive IP detected in Jurkat cells. Positive WB detected in Jurkat cells. Positive IHC detected in human lymphoma tissue. Observed molecular weight by Western-blot: 56 kDa
  • Tested applications
    ELISA, WB, IHC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    LCK antibody; LSK antibody; p56 LCK antibody; p56lck antibody; pp58lck antibody; Protein YT16 antibody; Proto oncogene Lck antibody; Tyrosine protein kinase Lck antibody; YT16 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of LCK recombinant protein (Accession Number: BC013200). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:112172(LCK antibody) at dilution of 1:500

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:112172(LCK antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:112172(LCK antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:112172(LCK antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:112172(LCK antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:112172(LCK antibody) at dilution of 1:100 (under 40x lens)

    IP Result of anti-LCK (IP:Catalog No:112172, 4ug; Detection:Catalog No:112172 1:500) with Jurkat cells lysate 4000ug.

    IP Result of anti-LCK (IP:Catalog No:112172, 4ug; Detection:Catalog No:112172 1:500) with Jurkat cells lysate 4000ug.

  • Background
    LCK(Lymphocyte cell-specific protein-tyrosine kinase) is also named as LSK,p56-LCK and a nonreceptor protein-tyrosine kinase of the SRConcogene family that is involved in transduction of T-cell receptor-mediated activation.It is implicated in the control of lymphocyte growth by virtue of its overexpression in some lymphoid malignancies and its transforming activity in heterologous systems(PMID:2784463).It has 3 isoforms produced by alternative splicing.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"