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Product Name
LARS2 antibody
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Description
LARS2 Rabbit Polyclonal antibody. Positive IP detected in Jurkat cells. Positive WB detected in HepG2 cells, Jurkat cells, MCF7 cells. Observed molecular weight by Western-blot: 102 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
KIAA0028 antibody; LARS2 antibody; Leucine tRNA ligase antibody; LEURS antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of LARS2 recombinant protein (Accession Number: NM_015340). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:112150(LARS2 antibody) at dilution of 1:400
IP Result of anti-LARS2 (IP:Catalog No:112150, 4ug; Detection:Catalog No:112150 1:500) with Jurkat cells lysate 2000ug.
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Background
LARS2 (Probable leucine--tRNA ligase, mitochondrial) is also named as mitochondrial LEURS. The deduced 903-amino acid protein has an N-terminal mitochondrial targeting signal that is cleaved after residue 39. LARS2 has characteristics of a class I aminoacyl-tRNA synthetase, including a classical Rossmann fold. It belongs to the class-I aminoacyl-tRNA synthetase family.
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References
- Perli E, Giordano C, Pisano A. The isolated carboxy-terminal domain of human mitochondrial leucyl-tRNA synthetase rescues the pathological phenotype of mitochondrial tRNA mutations in human cells. EMBO molecular medicine. 6(2):169-82. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"