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Product Name
LARS antibody
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Description
LARS Rabbit Polyclonal antibody. Positive IHC detected in human colon tissue, human brain tissue, human lung cancer tissue. Positive IF detected in HepG2 cells. Positive IP detected in Jurkat cells. Positive WB detected in Raji cells, A549 cells, HeLa cells, Jurkat cells, Y79 cells. Observed molecular weight by Western-blot: 135-140 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ10595 antibody; FLJ21788 antibody; hr025Cl antibody; HSPC192 antibody; KIAA1352 antibody; LARS antibody; LARS1 antibody; Leucine tRNA ligase antibody; leucyl tRNA synthetase antibody; LEURS antibody; LEUS antibody; LRS antibody; PIG44 antibody; RNTLS antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of LARS recombinant protein (Accession Number: NM_020117). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Raji cells were subjected to SDS PAGE followed by western blot with Catalog No:112149(LARS antibody) at dilution of 1:2000
Immunohistochemical of paraffin-embedded human colon using Catalog No:112149(LARS antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon using Catalog No:112149(LARS antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using LARS antibody Catalog No:112149 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-LARS (IP:Catalog No:112149, 4ug; Detection:Catalog No:112149 1:500) with Jurkat cells lysate 2400ug.
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Background
LARS represents leucyl-tRNA synthetase
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References
- Papageorgiou A, Rapley J, Mesirov JP, Tamayo P, Avruch J. A genome-wide siRNA screen in mammalian cells for regulators of S6 phosphorylation. PloS one. 10(3):e0116096. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"