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Product Name
LAMB1 antibody
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Description
LAMB1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IP detected in NIH/3T3 cells. Positive WB detected in NIH/3T3 cells, Jurkat cells. Observed molecular weight by Western-blot: 220 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
CLM antibody; LAMB1 antibody; Laminin 1 subunit beta antibody; Laminin 10 subunit beta antibody; Laminin 12 subunit beta antibody; Laminin 2 subunit beta antibody; Laminin 6 subunit beta antibody; Laminin 8 subunit beta antibody; Laminin B1 chain antibody; Laminin subunit beta 1 antibody; laminin antibody; beta 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of LAMB1 recombinant protein (Accession Number: NM_002291). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IF: 1:20-1:200
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Validations
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:112270(LAMB1 antibody) at dilution of 1:1000
Immunofluorescent analysis of HepG2 cells, using LAMB1 antibody Catalog No:112270 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-LAMB1 (IP:Catalog No:112270, 4ug; Detection:Catalog No:112270 1:1000) with NIH/3T3 cells lysate 2000ug.
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Background
Laminins are a family of basement membrane glycoproteins composed of three nonidentical chains, laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) (PMID: 7959779). Laminins contribute to the structure of extracellular matrix and influence the behavior of associated cells, such as adhesion, differentiation, migration, phenotype stability, and resistance to anoikis (PMID: 23057746). LAMB1 (laminin, beta 1) is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin 1, the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. LAMB1 is also a subunit of laminin-2 (laminin-211 or merosin), laminin-6 (laminin-311 or K-laminin), laminin-8 (laminin-411), laminin-10 (laminin-511) and laminin-12 (laminin-213).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"