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Product Name
L2HGDH antibody
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Description
L2HGDH Rabbit Polyclonal antibody. Positive WB detected in mouse small intestine tissue, rat brain tissue, SGC-7901 cells. Positive IHC detected in human brain tissue. Observed molecular weight by Western-blot: 46-48 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
C14orf160 antibody; DURANIN antibody; FLJ12618 antibody; L2HGDH antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of L2HGDH recombinant protein (Accession Number: BC006117). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with Catalog No:112262(L2HGDH antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human brain using Catalog No:112262(L2HGDH antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:112262(L2HGDH antibody) at dilution of 1:100 (under 40x lens)
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Background
L2HGDH(L-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as duranin, C14orf160 and belongs to the L2HGDH family.T he putative L2HGDH is predicted to be targeted to the mitochondria where its mitochondrial targeting sequence is presumably removed(PMID:16005139). Defects in L2HGDH are the cause of L-2-hydroxyglutaric aciduria (L2HGA). It has 2 isoforms produced by alternative splicing with the molecular weight of 50 kDa and 48 kDa.
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References
- Intlekofer AM, Dematteo RG, Venneti S. Hypoxia Induces Production of L-2-Hydroxyglutarate. Cell metabolism. 22(2):304-11. 2015.
- Krell D, Assoku M, Galloway M, Mulholland P, Tomlinson I, Bardella C. Screen for IDH1, IDH2, IDH3, D2HGDH and L2HGDH mutations in glioblastoma. PloS one. 6(5):e19868. 2011.
- Shim EH, Livi CB, Rakheja D. L-2-Hydroxyglutarate: an epigenetic modifier and putative oncometabolite in renal cancer. Cancer discovery. 4(11):1290-8. 2014.
- Ma S, Jiang B, Deng W. D-2-hydroxyglutarate is essential for maintaining oncogenic property of mutant IDH-containing cancer cells but dispensable for cell growth. Oncotarget. 6(11):8606-20. 2015.
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