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Product Name
KRAS-2A-specific antibody
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Description
KRAS-2A-specific Rabbit Polyclonal antibody. Positive IF detected in SH-SY5Y cells. Positive IHC detected in human lung cancer tissue, human colon cancer tissue. Positive FC detected in HeLa cells. Positive WB detected in SH-SY5Y cells. Observed molecular weight by Western-blot: 21-24 kDa
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Tested applications
ELISA, WB, IHC, FC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
C K RAS antibody; c Ki ras antibody; GTPase KRas antibody; K Ras 2 antibody; K RAS2A antibody; K RAS2B antibody; K RAS4A antibody; K RAS4B antibody; KI RAS antibody; KRAS antibody; KRAS1 antibody; KRAS2 antibody; KRAS2A antibody; KRAS2B speicifc antibody; KRAS4A antibody; Krasa antibody; Krasb antibody; NS3 antibody; RASK2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_033360). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with Catalog No:112120(KRAS-2A-specific antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:112120(KRAS-2A-specific antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:112120(KRAS-2A-specific antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (-20oc Acetone) fixed SH-SY5Y cells using Catalog No:112120(KRAS-2A-specific Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 HeLa cells were stained with 0.2ug KRAS-2A-specific antibody (Catalog No:112120, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
RAS (derived from 'RAt Sarcoma' virus) gene family includes KRAS, HRAS, and NRAS. These genes encode very similar 21 kDa proteins and are homologs of rodent sarcoma virus genes that have transforming abilities. KRAS has two isoforms, 2A and 2B. This antibody is specific for KRAS-2A, which also named as KRAS-4A .
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"