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Product Name
KPNA2 antibody
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Description
KPNA2 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human colon cancer tissue. Positive WB detected in HepG2 cells, Jurkat cells, mouse heart tissue, Raji cells. Observed molecular weight by Western-blot: 55-58 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Importin subunit alpha 2 antibody; IPOA1 antibody; Karyopherin subunit alpha 2 antibody; KPNA2 antibody; QIP2 antibody; RAG cohort protein 1 antibody; RCH1 antibody; SRP1 antibody; SRP1 alpha antibody; SRP1alpha antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of KPNA2 recombinant protein (Accession Number: NM_002266). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:112113(KPNA2 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:112113(KPNA2 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:112113(KPNA2 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:112113(KPNA2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
KPNA2 represents karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
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References
- Wang CI, Chien KY, Wang CL. Quantitative proteomics reveals regulation of karyopherin subunit alpha-2 (KPNA2) and its potential novel cargo proteins in nonsmall cell lung cancer. Molecular & cellular proteomics : MCP. 11(11):1105-22. 2012.
- Wang CI, Wang CL, Wu YC. Quantitative proteomics reveals a novel role of karyopherin alpha 2 in cell migration through the regulation of vimentin-pErk protein complex levels in lung cancer. Journal of proteome research. 14(4):1739-51. 2015.
- Yoshitake K, Tanaka S, Mogushi K. Importin-α1 as a novel prognostic target for hepatocellular carcinoma. Annals of surgical oncology. 18(7):2093-103. 2011.
- Wang CI, Wang CL, Wang CW. Importin subunit alpha-2 is identified as a potential biomarker for non-small cell lung cancer by integration of the cancer cell secretome and tissue transcriptome. International journal of cancer. 128(10):2364-72. 2011.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"