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Product Name
KPNA1 antibody
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Description
KPNA1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells, MCF-7 cells. Positive IHC detected in human testis tissue, human kidney tissue, human ovary tissue, human placenta tissue. Positive IP detected in HEK-293 cells. Positive WB detected in K-562 cells, HepG2 cells, Jurkat cells. Observed molecular weight by Western-blot: 60 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Importin subunit alpha 1 antibody; IPOA5 antibody; Karyopherin subunit alpha 1 antibody; KPNA1 antibody; Nucleoprotein interactor 1 antibody; RAG cohort protein 2 antibody; RCH2 antibody; SRP1 antibody; SRP1 beta antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of KPNA1 recombinant protein (Accession Number: NM_002264). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:112112(KPNA1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human testis using Catalog No:112112(KPNA1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:112112(KPNA1 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using KPNA1 antibody Catalog No:112112 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-KPNA1 (IP:Catalog No:112112, 4ug; Detection:Catalog No:112112 1:1000) with HEK-293 cells lysate 1480ug.
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Background
KPNA1 represents karyopherin alpha 1 (importin alpha 5)
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References
- Moriyama T, Nagai M, Oka M, Ikawa M, Okabe M, Yoneda Y. Targeted disruption of one of the importin α family members leads to female functional incompetence in delivery. The FEBS journal. 278(9):1561-72. 2011.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"